Jun 30, 2010

Cleaning the room at the bottom

One of my relatives once asked me: “I saw a guy talk about nanotechnology in the TV and I was reminded of you. Is that what you do?” How could I explain that making metal oxide nanoparticles for solar cells was different from designing DNA motifs. I reluctantly said “Yes, but mine is slightly different”.

When we hear the word Nanotechnology, we get the feel that it is something new, something different. It is neither. Metal nanoparticles have been in the making for two decades and the vision of DNA nanostructures through self-assembly was seen as early as the early 80’s. It is not new. There are very few separate ‘centers’ or departments for nanotechnology in universities and research institutes, but the number is rising. But it does not reflect that actual nanotechnology research is just budding at these places. There are a lot more people working on it in the departments of physics, chemistry, biology, you name it! It is not different. So when people ask me about nanotechnology, I tell them it is the study of any material in the nanoscale. As of now, the relevance is mainly toward physical sciences as there are more applications we see and hear in the market. But in reality, it extends to a far wider spectrum.

It is only the buzz about Nanotechnology that has made this commotion. People should understand that it is the nature of every material to be in any form or any scale, and when we make or work on materials in the range of one billionth of a meter, that is where Nanotechnology steps in. You cannot study the properties of a ceramic material without knowing the physics behind its mechanical strength. You cannot design a drug-delivery nanoshell without understanding the physiological path it has to take to reach the particular cell in the body. How could a DNA nanostructure be designed without knowing the chemical stability of its twists and flips? It is Physics. It is Chemistry. It is biology. From what I understand, it always gives us a sense of elation when we tell people we do something different. Instead of ‘research in physics’, ‘I work in nanotechnology’ seems hi-fi. I agree it does. But you are ‘scientifically’ still in physics. Science has no boundaries. You cannot separate each field and remain in just one for too long. It is to our convenience that we name each area so that colleagues in that field would be benefitted in discussions, and references toward a particular research would be simpler.

I talked to my friend in Germany who works in NMR study of nanomaterials, and I could not comprehend the complete subject of his research. The same was true with his understanding of DNA, the field I work in. And we both can call ourselves researchers in nanotechnology. It is a wide field, and you could be anywhere, doing anything. Everyone who works in nanotechnology may/will/need not know what each other does, except his area of research of course. Understanding nanotechnology is not distinct from physics, biology or chemistry; it is time to understand that nanotechnology, in itself, is not a book, but just a page in the book called Science.

A friend of mine once asked me about a master’s program in nanotechnology. I asked him why he wanted to do it since he already had a master’s degree. His reply was that he wanted to do his PhD in Nanoscience. The area you choose for your research is your discretion, until the point it has some relevance with what you studied. You do not have to have a degree in nanoscience or nanotechnology to do your research in the field! If that were the case, how do we have so many institutes in nano when we did not have (or did not even hear) degree programs on nanotechnology and what did our professors specialize in?? A microbiologist could do a research in nanotechnology, so could a chemist. There are no restrictions or requirements.

It has become a trend to include the word (prefix, suffix) ‘nano’ in a research paper and a paper published with ‘nano’ in the title need not involve an actual work in Nanotechnology. On the contrary, any research in Nanotechnology does not have to have the prefix in its title. It will take some time for this craze to end, or submerge. For now, I would rather say I work in the department of Chemistry, specializing in DNA crystallography!

All said, when someone asks me if I’m doing my research in nanotechnology, I would simply say “Yes” and if they tell me they know someone who works in nanotechnology, I’d say “Oh, nice”. And there ends the story.

Nanotechnology, all in one, and one in all.

Jul 9, 2009

நானோ டெக்னாலஜி

டாக்டர் ஆல்பர்ட் ஐன்ஸ்டைன் தனது டாக்ரேட் ஆராய்ச்சிக் கட்டுரை-ஐ அளிக்கும் போது ஒரு சர்க்கரை மூலக்கூறின் கூட்டணுவின் பரிமாணத்தை சுமார் ஒரு நானோமீட்டர் என்று அறிவித்தார், முதன் முதலாக நானோமீட்டர் என்னும் அளவு உலகுக்கு அறிமுகமானது. நானோமீட்டர் என்பது ஒரு மீட்டரின் பில்லியன் பகுதி. ஒரு நானோமீட்டர் என்பது பத்து ஹைட்ரஜன் அணுக்களை பக்கத்தில் வைத்தால் கிடைக்கும் நீளம்." நானோ" உலகின் விதிகள், குவாண்டம் இயற்பியல் விதிகள். இவைகளை விளக்க புதிய முறைகள், கருவிகள் வேண்டும். அவைகளை சரியாக அறியும் போது வெளிப்படும் சாத்தியங்கள் மனிதனை கடவுளுக்கு அருகில் கொண்டுசெல்கின்றன. சுமார் நூறு நானோ மீட்டரில் இருந்து நானோ டெக்னாலஜி ஆரம்பிக்கிறது. படிப்படியாக அளவு குறைந்துகொண்டேபோய் ஒரு தனிப்பட்ட எலெக்ட்ரானை நம் விருப்பத்தை போல நடத்துவதுதான் இந்த இயலின் குறிக்கோள். ஆராய்ச்சி முறைகள் சில, " இது சாத்தியமே" என்கிற நம்பிக்கையை தருகின்றன. நானோ டெக்னாலஜின் ஆதார சாகசம் அணு அளவில் பொருள்களை நம் விருப்பதை போல மாற்றுவது. ஒரு பொருளின் அணுகட்டத்தை மாற்றினால் அந்தப் பொருளின் இயற்கை பண்புகள் மாறிவிடும் என்பதே இதன் அடிப்படை. உதாரணமாக, அடுபுக்கரியின் அணுகட்டமைப்பை சற்று மாற்றினால் அது வைரமாகிறது. இயற்கையில் இயல்பாக நடக்கும் இயற்கை வினைகளை ஒரு ஆராய்ச்சிசாலையில் மனிதன் செய்ய முடியும் என்று நம்பிக்கை தருகிறது இந்த டெக்னாலஜி. டெக்ஸ்லெர், மூலக்கூறு கம்ப்யூட்டர்களை வடிவமைபத்ற்கான யோசனை இயற்கையில் ப்ரோடீன் தயாரிப்பை கவனித்தால் கிடைக்கும் என்று சொல்கிறார். மேலும் ஒரு மரபணு அதற்குள்ளேயே அதன் இறுதி வடிவத்தின் செய்தியை வைத்திருப்பதை போல ஒரு கம்ப்யூட்டர் தான் இறுதியில் இந்தப் வேலை செய்யும் சாதனமாகப் போகிறோம் என்கிற அறிவை அதற்குள்ளே வைத்துவிட முடியும் என்கிறார். நூறு நானோ மீட்டரைவிட நுட்பமாக பொருள்களை தயாரிக்கும் திறமைதான் நானோ டெக்னாலஜி. இந்தத் திறமையை இரண்டு விதமாக அணுகுகிறார்கள், ஒன்று, "டாப் டவுன் " மற்றொன்று "பாட்டம் அப் ". அதாவது படிப்படியாக அளவை மாற்றிக்கொண்டே போய் "நானோ" அளவை எட்டுவது. இன்றைய தினத்தின் மைக்ரோ டெக்னாலஜி முறைகள் இந்த "டாப் டவுன்" வகையைச் சார்ந்தவை.மூலக்கூறுகள் என்னும் கூட்டணுவை ஒரு ச்விட்சாக மாற்றலாம் என்கிற யோசனை சுமார் 25 வருடங்களாக இருக்கிறது. இது சமிபத்தில் தான் நடைமுறையில் சில வேதியியல் பொறியியல் வளர்ச்சிகளினால் சாத்தியமாகி இருக்கிறது. மூலக்கூறுகள் என்னும் கூட்டணுக்கள் சில சேர்ந்து கொண்டு oxidation reduction என்னும் வேதியியல் மாற்றம் பெறும் போது ஒரு ச்விட்சாக இயங்குகிறது என்று UCLA பல்கலைகழகத்திலும், ஹியூலிட் பக்கர்டு நிறுவனத்திலும் ஆராய்ச்சியாளர்கள் கண்டுள்ளனர். நானோ டெக்னாலஜியின் பொருள்கள் வாயு அல்லது திரவ வடிவில் இல்லாமல் அதே சமயம் திடப்பொருள்கள் போல திடமாக இல்லாமல் குறிப்பிட்ட வரிசையில் மாறக்கூடிய திடப்பொருள்கள். நானோ டெக்னாலஜியின் தயாரிப்பு முறையில், அணு அளவில் மாற்றங்கள் நிகழ்த்தப்படுகின்றன. ஒரு பொருளை அணு அணுவாக, சீராக உற்பத்தி செய்ய முடியும் என்பது சாத்தியம் எனில் கழிவுப் பொருள்கள் இருக்காது. தொழிற்சாலைகளில் விஷ வாயுக்களை காற்றில் கலக்காமல் செயல்படுத்தலாம். விரயமான செயல்கள் இல்லாததால் ஆற்றல் அதிகரிக்கும்.
இன்றைய இந்தத் தொழில்நுட்பம் ஓர் அபரிவிதமான வளர்ச்சியை மனித வாழ்வில் கொண்டுவரும் என்பதில் சந்தேகமில்லை.

Dec 5, 2007

Stem Cells Sans Embryos

Stem cells with the capacity to form any type of tissue can be created from adult cells without destroying embryos, according to a new research that suggests a way of sidestepping ethical controversy over the field.

Embryonic stem cells (ESCs) are found only in early embryos and are attractive to medical researchers as they are ‘pluripotent’ – they can give rise to all the 200 or so tissue types found in the human body. The goal is to find ways of directing their growth as they can be used to provide replacement tissue for treating conditions such as Parkinson’s, diabetes, and spinal paralysis. They are also the source of ethical controversy: the cells are derived from excess human embryos discarded after in vitro fertilization and obtaining them requires destruction of the embryos.

Two groups – one led by Shinya Yamanaka at Kyoto university in Japan and one led by Thompson and Junying Yu at the University of Wisconsin – separately engineered human skin cells to express 4 different genes. For reasons not yet clear, exposing cells to these genes appears to turn back the developmental clock. Both groups found that the resulting cells exhibit two major properties that define ESC’s – pluripotency divide indefinitely in their undifferentiated state. While the new cells look and act like ESCs, it is not yet clear just how similar they are. A screen of the expression of 30,000 genes showed that the pluripotent cells are similar but not identical to ESCs.

Both teams used viral DNA to introduce genes for 4 transcription factors – proteins that turn on other genes in the cell – into fibroblasts – a type of skin cell. Two of these transcription factors were the same in both groups; two were different. Scientists theorize that when expressed in adult cells. The transcription factors activate a genetic cascade that returns the cell and its DNA to an embryonic–like state. When implanted into mice, the cells generated a ball of tissue containing multiple differentiated cell types, a standard test for cell pluripotency. Yamanaka’s team also showed that the cells could differentiate into muscle and nerve cells, employing the same protocols used with ESCs.

Before these cells can be considered for human therapeutics, the researchers will need to develop an alternative way to express the transcription factors. The viruses currently used can integrate into genome and pose potential safety concerns. Germline transmission is the final and definitive proof that these cells can do anything a traditionally derived ESC can do.

There is still a long way to go, however!

Source:
http://www.technologyreview.com/biotech/19742
http://www.timesonline.co.uk/tol/news/uk/health/article1895546.ece

Nov 24, 2007

Life minus Nine

We define life in many ways. All the research in the world is aimed primarily at only one target - improving human lives. The past steps lead us to a new gate called ‘nanotechnology'. Although research in this field has been going on for decades, it is only now that the focus light is turned on. It will be worthwhile to spend some time on it.

Nanotechnology, like life, can be defined in many ways, its areas extending far beyond just physics, chemistry, or biology. Here, I try to relate these hard-to-define terms, "life" and "nano". What would life be 'minus nine'? I would rather prefer to talk about life 'plus' nine.

It is customary to define nanoparticles or nanostructures as entities in the range of sizes from 1 - 100 nm, thus many biological materials can be classified as nanoparticles. Considering a gradation in this range, we can include, viruses, which range in size between 10 to 200 nm, in the upper part of the nanoparticle range. Proteins, ranging between 4 and 50 nm, are in the low nanometer range, while the building blocks of proteins, the amino acids - each about 0.6 nm in size - are below the lower limit of a nanoparticle. These are a few of the examples that could be considered in the nanoscale. The structures made up by these particles sometimes end up in the same range too.

A protein is a combination of any of the 20 amino acids, bound together one after the other by strong peptide chemical bonds. These chains called polypeptides contain hundreds, and in some cases thousands of amino acids; hence they correspond to "nanowires". The polypeptide nanowires further undergo twistings and turnings to compact themselves into a relatively small volume forming a polypeptide nanoparticle, with a diameter that is typically in the range of 4 - 50 nm. Thus a protein is a nanoparticle consisting of a compacted polypeptide nanowire.

The genetic material deoxyribonucleic acid (DNA) has the structure of a compacted nanowire. It is made up of 4 nucleotide molecules that bind together in a long double helix to form chromosomes. Thus the DNA molecule is a double-nanowire, with the strands twisted around each other with a repeat unit every 3.4 nm, and a diameter of 2 nm.

Another biological structure made up of subunits in the nanometer range size is the human tendon. The function of a tendon is to attach a muscle to a bone. The fundamental building block of a tendon is the assemblage of amino acids that form a gelatin- like protein called 'collagen' (1 nm), which coils into a triple helix (2 nm). Further arrangement then follows a three-fold sequence of fibrillar nanostructures: a microfibril (3 - 5 nm), a subfibril (10 - 20 nm), and a fibril itself (50 - 500 nm). These nanostructures then make up the macroscopic tendon.

A view of these biological nanostructures would give us an idea what role the study of nanoparticles plays in biology. Taken to the scrap, many important biomolecules may end up in nanoparticles. Since the smallest amino acid, glycine is 0.42 nm in size, and some viruses reach 200 nm, it will be appropriate to define a biological nanostructure as being in the nominal range from 0.5 to 200 nm. It is our discretion to study them as a separate class, to place them in a separate group, or to create a new field called 'nanobiotechnology'.

Remember, best things come in small packages!

Oct 7, 2007

Take a closer look




This is perhaps the smallest toilet in the world!
This nanotoilet is the winner of the Most Bizarre prize at The 49th International Conference on Electron, Ion and Photon Beam Technology and Nanofabrication Bizarre/Beautiful Micrograph Contest.

Here is the 2004 winner...




Fishing with Nanotubes – These micro fish appear hooked on nanotubes with a catalyst bait.
Check out the rest of the images here.

Oct 2, 2007

Flying Nano Wonders

Butterflies are probably the most beautiful creatures on earth. Those colorful wings captivates you with its beauty.

Have you ever thought whether those magical patterns are formed by true colors??? In fact, these clever insects trick you into believing that their wings possess true colors. The wings have what can be called biophotonic crystals that scatter light in some intriguing ways. Something our 21st century physicists have hard time replicating in labs!

These are some ridges of nano dimensions found on the surface of the wings. These creatures have been exploiting the potentials of nanotechnology for the past 30 million years!!!!

What you see here is not some fabric... but the wings of a butter fly.



I found more details about this here. You may want to take a look at this site!

Nano in the Eyes

At John Hopkins, researchers have developed a nanoparticle as a new biosensor to treat damaged retinal cells.

Biosensor detects oxidative stress inside the cells. It responds by regulating therapeutic gene expression. This reduces the damage caused by the free radicals.

Biosensor has an outer lipid layer. This layer also has some targeting proteins. These proteins recognize the endothelial cells lining the eye's blood vessels. The lipid outer layer ensures easy entry of the biosensor into the cell.

Once inside the cells, the DNA is exposed. If there is oxidative stress, the promoters are activated and two proteins are expressed. One, a fluorescent protein that helps in detection of the damaged cells; two, a therapeutic protein that reduces the damage.

They have tested the biosensor in animal models like mouse, rabbits, and dogs. These nanoparticles were also found to be nontoxic on cells but animals showed varied levels of toxicity.

You will find more info here.

Toxin - Nanoparticle Combine Defeats Cancer

Diptheria toxin is a protein secreted by Corynebacterium diphtheriae.



The protein has three domains - A, B and T. The B domain is believed to be responsible for recognizing the cell surface receptors. The toxin is found to show affinity for heparin binding epidermal like growth factor like precursors, which are found on the cell surface. T domain aids the protein to gain entry into the cell.



The A chain has the enzymatic activity. It catalyzes the ADP ribosylation of eukaryotic aminoacyl transferase II. This prevents protein biosynthesis in the cells leading to cell death.

The diphtheria toxin A (DT - A) chain's toxic properties can be exploited by properly guiding them to the cells that need to be killed.

This is exactly what the researchers have done using nanotechnology.

A nanoparticulate system made of Poly(beta amino ester) polymer and poly( butane diol diacrylate co amino pentanol) was used to deliver the diphtheria toxin A (DT - A) gene. This gene is linked to a regulatory sequence that gets activated only in prostate cancer cells. While the specificity is taken care of by the gene regulator, safe delivery of the toxin into these cells is the responsibility of the nanoparticulate system. Now, the diphtheria toxin does the rest.

Studies in the laboratory show promising results. Well, I guess we can expect efficient nano based injectable or ingestible drugs to treat cancer in the near future.